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Promega ribo m7g cap analog
Ribo M7g Cap Analog, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ribo+m7g+cap+analog/ribo+m7g+cap+analog/pmc10288568__pnas__2221313120__sapp-72-24-28
Average 90 stars, based on 1 article reviews
ribo m7g cap analog - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Virus:


Recombinant:


Protease Inhibitor:


RNA Extraction:


Real-time Polymerase Chain Reaction:


SYBR Green Assay:


Protein Purification:


Plasmid Preparation:

Article Title: Blood-to-Testis Transport of Ribavirin Involves Carrier-Mediated Processes at the Blood-Testis Barrier.
Article Snippet: TaggedAPTARAEndTaggedAPTARAEnd bbreviations: BTB, blood−testis barrier; CNT, concent DMSO, dimethyl sulfoxide; ECF, extracellular fluid; EN transporter; ENT, equilibrative nucleoside transporter; H methyl-4-phenylpyridinium; NBMPR, S-[(4-nitrophenyl) L-carnitine/organic cation transporter; PCR, polymerase deviation; siRNA, small interfering RNA; SLC, solute carr nucleobase transporter.. TaggedAPTARAEndTaggedAPTARAEnd* Corresponding author.. E-mail address: kubo.yoshiyuki.jf@teikyo-u.ac.jp (Y. K

Synthesized:

Article Title: Blood-to-Testis Transport of Ribavirin Involves Carrier-Mediated Processes at the Blood-Testis Barrier.
Article Snippet: TaggedAPTARAEndTaggedAPTARAEnd bbreviations: BTB, blood−testis barrier; CNT, concent DMSO, dimethyl sulfoxide; ECF, extracellular fluid; EN transporter; ENT, equilibrative nucleoside transporter; H methyl-4-phenylpyridinium; NBMPR, S-[(4-nitrophenyl) L-carnitine/organic cation transporter; PCR, polymerase deviation; siRNA, small interfering RNA; SLC, solute carr nucleobase transporter.. TaggedAPTARAEndTaggedAPTARAEnd* Corresponding author.. E-mail address: kubo.yoshiyuki.jf@teikyo-u.ac.jp (Y. K

Extraction:

Article Title: Blood-to-Testis Transport of Ribavirin Involves Carrier-Mediated Processes at the Blood-Testis Barrier.
Article Snippet: TaggedAPTARAEndTaggedAPTARAEnd bbreviations: BTB, blood−testis barrier; CNT, concent DMSO, dimethyl sulfoxide; ECF, extracellular fluid; EN transporter; ENT, equilibrative nucleoside transporter; H methyl-4-phenylpyridinium; NBMPR, S-[(4-nitrophenyl) L-carnitine/organic cation transporter; PCR, polymerase deviation; siRNA, small interfering RNA; SLC, solute carr nucleobase transporter.. TaggedAPTARAEndTaggedAPTARAEnd* Corresponding author.. E-mail address: kubo.yoshiyuki.jf@teikyo-u.ac.jp (Y. K

Sequencing:

Article Title: A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Antibodies GST-tag Antibody [HRP] GenScript A00130 His-tag Antibody, Mouse GenScript A00186 Mouse anti GFP-tag ABclonal AE012 Mouse anti DDDDK-Tag ABclonal AE005 Oligonucleotides and other sequence-based reagents PCR and qRT-PCR primers This study Table EV 1 Chemicals, enzymes, and other reagents NaCl Sigma-Aldrich 31434 NaOH Sigma-Aldrich 221465 Salicylic acid Sigma-Aldrich W398500 Agarose Sigma-Aldrich A0576 Reagent/resource Reference or source Identifier or catalog number KCl Sigma-Aldrich P9541 Na2SO4 Sigma-Aldrich 238597 MgCl2 Sigma-Aldrich M2393 CaCl2 Sigma-Aldrich C3306 HEPES Sigma-Aldrich H3375 MES Sigma-Aldrich 475893 Tetracycline Sigma-Aldrich 58346-M Streptomycin sulfate Sigma-Aldrich 5711 Bis-Tris propane Sigma-Aldrich 64431-96-5 Na15NO3 Sigma-Aldrich 364606 NaNO3 Sigma-Aldrich S8170 SD-Leu/Trp Coolaber PM2221 SD-Leu/Trp/His/Ade Coolaber PM2112 Tris-HCl Sigma-Aldrich 10812846001 EDTA Sigma-Aldrich 4005-OP Dimethyl sulfoxide (DMSO) Sigma-Aldrich D8418 D-Mannitol Sigma-Aldrich 1371621000 PEG3350 Sigma-Aldrich P4338 PBS Sigma-Aldrich P3813 T7 RiboMAX large-scale RNA production system Promega P1300 Ribo m7G Cap Analog Promega P1711 © The Author(s) The EMBO Journal 11 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number NheI Thermo Fisher FD0947 SphI Thermo Fisher FD0604 RNA isolater Total RNA Extraction Reagent Vazyme R401-01 HiScript II Q RT SuperMix for qPCR Vazyme R223-01 Hieff qPCR SYBR Green Master Mix YESEAN 11201ES Salmon Sperm DNA Solution Thermo Fisher 15632011 Glutathione Resin GenScript L00206 Software GraphPad Prism 8.0 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html Other Hiseq 2500 platform Illumina Plant materials and growth conditions For the GWAS, we obtained 198 soybean accessions (Zhang et al, 2021) from Jiangsu Academy of Agricultural Sciences, and grew them in a culture room at 25 °C under long-day (16 h light/8 h dark) conditions.

Polymerase Chain Reaction:

Article Title: A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Antibodies GST-tag Antibody [HRP] GenScript A00130 His-tag Antibody, Mouse GenScript A00186 Mouse anti GFP-tag ABclonal AE012 Mouse anti DDDDK-Tag ABclonal AE005 Oligonucleotides and other sequence-based reagents PCR and qRT-PCR primers This study Table EV 1 Chemicals, enzymes, and other reagents NaCl Sigma-Aldrich 31434 NaOH Sigma-Aldrich 221465 Salicylic acid Sigma-Aldrich W398500 Agarose Sigma-Aldrich A0576 Reagent/resource Reference or source Identifier or catalog number KCl Sigma-Aldrich P9541 Na2SO4 Sigma-Aldrich 238597 MgCl2 Sigma-Aldrich M2393 CaCl2 Sigma-Aldrich C3306 HEPES Sigma-Aldrich H3375 MES Sigma-Aldrich 475893 Tetracycline Sigma-Aldrich 58346-M Streptomycin sulfate Sigma-Aldrich 5711 Bis-Tris propane Sigma-Aldrich 64431-96-5 Na15NO3 Sigma-Aldrich 364606 NaNO3 Sigma-Aldrich S8170 SD-Leu/Trp Coolaber PM2221 SD-Leu/Trp/His/Ade Coolaber PM2112 Tris-HCl Sigma-Aldrich 10812846001 EDTA Sigma-Aldrich 4005-OP Dimethyl sulfoxide (DMSO) Sigma-Aldrich D8418 D-Mannitol Sigma-Aldrich 1371621000 PEG3350 Sigma-Aldrich P4338 PBS Sigma-Aldrich P3813 T7 RiboMAX large-scale RNA production system Promega P1300 Ribo m7G Cap Analog Promega P1711 © The Author(s) The EMBO Journal 11 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number NheI Thermo Fisher FD0947 SphI Thermo Fisher FD0604 RNA isolater Total RNA Extraction Reagent Vazyme R401-01 HiScript II Q RT SuperMix for qPCR Vazyme R223-01 Hieff qPCR SYBR Green Master Mix YESEAN 11201ES Salmon Sperm DNA Solution Thermo Fisher 15632011 Glutathione Resin GenScript L00206 Software GraphPad Prism 8.0 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html Other Hiseq 2500 platform Illumina Plant materials and growth conditions For the GWAS, we obtained 198 soybean accessions (Zhang et al, 2021) from Jiangsu Academy of Agricultural Sciences, and grew them in a culture room at 25 °C under long-day (16 h light/8 h dark) conditions.

Quantitative RT-PCR:

Article Title: A phosphorylation-regulated NPF transporter determines salt tolerance by mediating chloride uptake in soybean plants.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Antibodies GST-tag Antibody [HRP] GenScript A00130 His-tag Antibody, Mouse GenScript A00186 Mouse anti GFP-tag ABclonal AE012 Mouse anti DDDDK-Tag ABclonal AE005 Oligonucleotides and other sequence-based reagents PCR and qRT-PCR primers This study Table EV 1 Chemicals, enzymes, and other reagents NaCl Sigma-Aldrich 31434 NaOH Sigma-Aldrich 221465 Salicylic acid Sigma-Aldrich W398500 Agarose Sigma-Aldrich A0576 Reagent/resource Reference or source Identifier or catalog number KCl Sigma-Aldrich P9541 Na2SO4 Sigma-Aldrich 238597 MgCl2 Sigma-Aldrich M2393 CaCl2 Sigma-Aldrich C3306 HEPES Sigma-Aldrich H3375 MES Sigma-Aldrich 475893 Tetracycline Sigma-Aldrich 58346-M Streptomycin sulfate Sigma-Aldrich 5711 Bis-Tris propane Sigma-Aldrich 64431-96-5 Na15NO3 Sigma-Aldrich 364606 NaNO3 Sigma-Aldrich S8170 SD-Leu/Trp Coolaber PM2221 SD-Leu/Trp/His/Ade Coolaber PM2112 Tris-HCl Sigma-Aldrich 10812846001 EDTA Sigma-Aldrich 4005-OP Dimethyl sulfoxide (DMSO) Sigma-Aldrich D8418 D-Mannitol Sigma-Aldrich 1371621000 PEG3350 Sigma-Aldrich P4338 PBS Sigma-Aldrich P3813 T7 RiboMAX large-scale RNA production system Promega P1300 Ribo m7G Cap Analog Promega P1711 © The Author(s) The EMBO Journal 11 D ow nloaded from https://w w w .em bopress.org on January 7, 2025 from IP 2400:1a00:b030:5c8c:1ec:4998:fda6:5e7. .. Reagent/resource Reference or source Identifier or catalog number NheI Thermo Fisher FD0947 SphI Thermo Fisher FD0604 RNA isolater Total RNA Extraction Reagent Vazyme R401-01 HiScript II Q RT SuperMix for qPCR Vazyme R223-01 Hieff qPCR SYBR Green Master Mix YESEAN 11201ES Salmon Sperm DNA Solution Thermo Fisher 15632011 Glutathione Resin GenScript L00206 Software GraphPad Prism 8.0 https:// www.graphpad.com ImageJ https:// imagej.nih.gov/ij/ index.html Other Hiseq 2500 platform Illumina Plant materials and growth conditions For the GWAS, we obtained 198 soybean accessions (Zhang et al, 2021) from Jiangsu Academy of Agricultural Sciences, and grew them in a culture room at 25 °C under long-day (16 h light/8 h dark) conditions.

In Vitro:

Article Title: The nitrate transporter NRT2.1 directly antagonizes PIN7-mediated auxin transport for root growth adaptation
Article Snippet: .. After linearization of pGEMHE plasmids by NheI digestion, cRNA was transcribed in vitro with a Ribo MAXTM Large Scale RNA Production System-T7 (Promega) and Ribo m7G Cap Analog (Promega). ..

Article Title: The triglyceride-synthesizing enzyme diacylglycerol acyltransferase 2 modulates the formation of the hepatitis C virus replication organelle.
Article Snippet: We terminated the transcription by addition of 3.25 U of RNase free DNase (NEB #M0303) per μg of plasmid DNA and incubation for 30 min at 37 ̊C. .. We extracted the RNAs using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel #740948.250), eluted them in RNAse free water and stored them at—80 ̊C after concentration determination with a spectrophotometer (Implen). pIRF1b [38] IVTs were generated as described above but with an additional capping step during the in vitro transcription using the Ribo m7G Cap Analog (Promega #P1711). ..

Concentration Assay:

Article Title: The triglyceride-synthesizing enzyme diacylglycerol acyltransferase 2 modulates the formation of the hepatitis C virus replication organelle.
Article Snippet: We terminated the transcription by addition of 3.25 U of RNase free DNase (NEB #M0303) per μg of plasmid DNA and incubation for 30 min at 37 ̊C. .. We extracted the RNAs using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel #740948.250), eluted them in RNAse free water and stored them at—80 ̊C after concentration determination with a spectrophotometer (Implen). pIRF1b [38] IVTs were generated as described above but with an additional capping step during the in vitro transcription using the Ribo m7G Cap Analog (Promega #P1711). ..

Spectrophotometry:

Article Title: The triglyceride-synthesizing enzyme diacylglycerol acyltransferase 2 modulates the formation of the hepatitis C virus replication organelle.
Article Snippet: We terminated the transcription by addition of 3.25 U of RNase free DNase (NEB #M0303) per μg of plasmid DNA and incubation for 30 min at 37 ̊C. .. We extracted the RNAs using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel #740948.250), eluted them in RNAse free water and stored them at—80 ̊C after concentration determination with a spectrophotometer (Implen). pIRF1b [38] IVTs were generated as described above but with an additional capping step during the in vitro transcription using the Ribo m7G Cap Analog (Promega #P1711). ..

Generated:

Article Title: The triglyceride-synthesizing enzyme diacylglycerol acyltransferase 2 modulates the formation of the hepatitis C virus replication organelle.
Article Snippet: We terminated the transcription by addition of 3.25 U of RNase free DNase (NEB #M0303) per μg of plasmid DNA and incubation for 30 min at 37 ̊C. .. We extracted the RNAs using the NucleoSpin RNA Clean-up Kit (Macherey-Nagel #740948.250), eluted them in RNAse free water and stored them at—80 ̊C after concentration determination with a spectrophotometer (Implen). pIRF1b [38] IVTs were generated as described above but with an additional capping step during the in vitro transcription using the Ribo m7G Cap Analog (Promega #P1711). ..

other:

Article Title: Integration of multi-omics data accelerates molecular analysis of common wheat traits
Article Snippet: After incubation for 20 h in the dark at 22 °C, confocal microscopy (Carl Zeiss, LSM710) was used to examine the protoplasts.



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